Friday, March 11, 2016

The PCR technique probably does not measure a viral load


What the PCR technique is

theperthgroup.com/RESPONSE/PGAffidavit.pdf

According to the HIV/AIDS experts HIV is a retrovirus with a unique RNA genome. The term genome is defined as the full complement of genes and the genome is necessary for the HIV particle to reproduce the virus particles.

Experts claim they are able to determine the number of RNA molecules in a specimen of blood using several methodologically different tests based on a biochemical technique known as the polymerase chain reaction (PCR).

The PCR is a technique which utilises a small piece of the DNA of interest to quickly multiply and detect the same DNA if present in test material.

In the case of retroviruses the DNA is obtained by reverse transcribing the retroviral RNA into DNA. DNA is used because the PCR technique only works with DNA, it doesn’t work with RNA.

Experts refer to the number obtained by the PCR tests as the “HIV “viral load” and state such measurements are essential for the clinical management of patients who are HIV positive.

The “viral load” is said to be the most reliable prognostic indicator for HIV infected individuals and is also said to guide the choice and determine the effectiveness of “antiretroviral” drug therapy.


The PCR technique may not measure a viral load

theperthgroup.com/RESPONSE/PGAffidavit.pdf

To identify RNA as that of a retrovirus a scientist must first purify the viral particles. This is because the cells in which viruses replicate also contain RNA. Since the particles said to be “HIV” have not been purified then it is not possible to claim a particular RNA is that of “HIV”.

There are no published correlations between the “viral load” (number of RNA molecules) and the number of particles considered to be “HIV” in blood. This is because to date no HIV researcher has published even one electron micrograph demonstrating the existence of even one such particle in the blood of even one AIDS patient.

Hence the term “viral load” is both unfounded and misleading.

In order to count RNA molecules a scientist must have a test able to distinguish between “HIV” RNA and all other RNAs. If, as HIV experts assert, the viral load measures “HIV” RNA, then this test must be capable of distinguishing between “HIV” RNA and all other RNAs. That is, by recognising “HIV” RNA, ipso facto the test proves HIV infection.

However, according to the US Centers for Disease Control (CDC), “In adults, adolescents, and children infected by other than perinatal exposure, plasma viral RNA nucleic acid tests should NOT be used in lieu of licensed HIV screening tests.

According to HIV experts, the role of “viral load” tests is confined to measuring the “quantity of virus” in patients whose “HIV” infection has first been proven by antibody tests.

Hence the test that the HIV experts assert able to count “HIV” specific RNA molecules is not considered capable of diagnosing “HIV” infection.

I conclude these tests are meaningless in terms of their ability to identify RNA as “HIV” let alone measure the “viral load”.

HIV experts acknowledge there are problems measuring the actual “viral load”. Different laboratories and different PCR tests obtain markedly different results for the same “viral load” on the identical specimens. Inter-laboratory and inter-test variability is used to justify expert recommendations that patients should always be tested by the same laboratory using the same assay. In other words, HIV experts are not concerned with the actual value of “viral load”. This leads one to question how is it possible (a) to make general, categorical statements about the biological relevance of “viral load”; (b) transpose such statements to individual patients whose “viral load” is being monitored for the same purposes. That is, making management decisions in regard to “antiretroviral” therapy and advising on prognosis.


What the PCR technique would be actually measuring

bmj.rethinkers.net/response_44053.html

The oxidising agents, to paraphrase Barbara McClintock, cause a "shock" that forces the genome to restructure itself in order to overcome a threat to its survival", thus leading to the appearance of novel non-viral RNAs, which may be detected with PCR.